Over the 4.6 billion years of Earth's history, microorganisms have existed for at least 3.4 billion years and are known as the "rulers of the Earth". For most of that time, humans and microorganisms have lived in harmony. However, there is a category of microorganisms that can invade the human body and cause infections and even epidemics.These microorganisms are known as pathogenic microorganisms or pathogens, including fungi, bacteria, mycoplasmas, chlamydia, and viruses. Among them, bacteria and viruses are the most harmful. The timely identification of these pathogenic microorganisms is a crucial step in clinical treatment.
through the identification of the morphological structure of pathogenic organisms, or staining and cultivation of pathogenic microorganisms, and based on this, carrying out biological identification in order to distinguish different types of microorganisms.Traditional detection methods mainly include smear microscopy, isolation and cultivation with biochemical reactions, and tissue cell culture;
Serological testing is a technique for detecting the antigen or antibody of the pathogen with known antibodies or antigens, thereby quickly identifying the pathogen. Common methods include serum agglutination technology, latex agglutination assay, fluorescence antibody detection technology, coagulation agglutination test, enzyme-linked immunosorbent assay and other technologies;
With the development of molecular biology, the identification of pathogenic microorganisms has also moved from morphological and biochemical levels to the molecular level. This detection method avoids the problems of long detection period, complex operation, limited sensitivity and specificity encountered in the first two methods. The fluorescence quantitative PCR detection technology is currently the most commonly used nucleic acid detection technology in practice, but it relies on amplification curve and Ct value to determine the result, which is easily affected by PCR inhibitors and amplification efficiency, and can only be relatively quantified with certain limitations in repeatability of the results.
As the third-generation PCR technology, digital PCR randomly divides the fluorescence quantification reaction system into tens of thousands of independent micro-reaction units based on single-molecule template PCR amplification for absolute quantification of nucleic acid copy number. The automatic liquid droplet digital PCR instrument independently developed by Borisui Biotechnology has the advantages of integration, full automation, and full closure in nucleic acid detection of pathogenic microorganisms,
which has the following advantages:
1）high sensitivity, up to 0.01%~0.001%;
2）no standard product and standard curve are needed;
3）absolute quantification can be achieved;
4）high tolerance to amplification inhibitors;
5）high repeatability.
6) It can be widely used in rapid detection of pathogenic microorganisms, dynamic monitoring, and medication guidance.
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